Canon non-binary characters

Welcome to FYME’s list of Canon Non-Binary Characters! This list is a work in progress, as mod Cory finds more characters and media to share. Please let me know if there are any characters that should be added!

A few disclaimers: non-binary characters aren’t always portrayed positively, or they are written using language that isn’t sensitive to our real experiences as non-binary people. But, members of marginalized groups sometimes connect with bad representation, and some of us take those characters and create something positive. So as many non-binary characters as I can find will be on this list. These are not necessarily recommendations.

A new disclaimer: Cory has not reviewed all of the sources of information here. I’m taking suggestions from followers as for what to add. The non-binary status of some characters may be open to interpretation, and the “canonness” of it could be up for debate. Other than in webcomics, we rarely get confirmation from creators about whether a character with ambiguous or non-conforming gender is actually non-binary, so we are using a loose definition of “canon.” Also, I cannot vouch for the content of all of the comics/books/etc listed.

Also, I made this gif-heavy. I hope that won’t cause any problems.

So now let’s get to the list:

Cartoons/TV

Apos: from the anime RIN ~Daughter of Mnemosyne~. Apos is the primary antagonist, an intersex angel who believes themself to be a god. (h-slur cw at the links)
Baron Ashura: from anime Mazinger Z, they were a cyborg created using the remains of a Mycenaean couple.
Berg Katse: from Science Ninja Team Gatchaman, they are a villain who was created by fusing fraternal twins in the womb. Katse changes gender almost every year.
BMO: A sentient robot from Adventure Time whose pronouns and gender presentation is fluid. (pictured above)
The Crystal Gems: From Steven Universe, the gender of the gems is basically “gem.” In fact, all gems introduced are non-binary
Envy: a shape-shifting Homunculus from Fullmetal Alchemist with no gender.
Kayli: from @mistlandscomic uses Xie pronouns and is made of stardust.
Neferpitou: from Hunter X Hunter is a cat-humanoid Chimera Ant. Pronouns are not used when referring to Neferpitou.
Piccolo Daimao (King Piccolo) and other Namekians: from the Dragon Ball series, Piccolo Daimao was an alien and the main villain. He has many children, which are all produced asexually.All Namekians appear “masculine” but they are all the same gender (h-slur cw at links).
Puppy: from Aerial Magic is a genderless spirit.
S. LaFontaine: Mad Scientist from Carmilla, comes out as non-binary through the course of the series. (the ginger in the second gif above)
Star Trek: There are several alien races in Star Trek canon that are non-binary, and there were a few characters that were the focus of individual episodes (often with tragic stories). For the most part, Star Trek discussions of gender has a strong focus on how these different genders procreate. There are no trans characters in Star Trek canon.

Andorians: This race was revealed to have four genders in DS9 novels. Humans refer to two of the genders as female, and two of them as male.
Axanar: an androgynous race introduced in Enterprise ep Fight or Flight. The episode did not focus on their gender.
J’naii: Their race once had two sexes, but they evolved to be a genderless, androgynous race. Soren was the focus of a TNG episode called The Outcast. She identified as female and was forced into conversion therapy (the closest we got to a trans character).
Trill: is a species that lives in humanoid hosts. When their hosts die, they will move to a new host, frequently changing gender through their lives. Odan was the focus of TNG ep The Host, in which they moved from a male to a female host (also, Odan is part of a sub-group of Trill with ridges, but most Trill have spots on their faces).
Vissians: a race that has three genders: male, female, and cogenitor. The cogenitors are treated more like animals than people. The focus of the Enterprise episode, Cogenitor, has a tragic ending.

Stevonnie: Also form Steven Universe, Stevonnie is a fusion of the half-gem/half-human Steven and of the human Connie. Creators of the show confirmed Stevonnie uses they/them pronouns. (pictures in the first gif)
Unity: from Rich and Morty is a collective hivemind. It uses both male and female avatars (cory does not recommend this show).
Yivo: A sentient, extraterrestrial being from Futurama. Shkle has shkler own set of pronouns.

Webcomics

Aderyn Orion Icarus: Protagonist of Streetcrows. The comic includes several other non-binary and binary trans characters.
The Alchemist: The mysterious villain of Agents of the Realm (@agentsoftherealm). Referred to with they pronouns.
Angellus: from @christis-comic: a 17 year old nephilim (half angel) who is non-binary and goes by he/they pronouns. The comic also includes:

Adrian: 18 year old gender non-conforming Devil prince, he/him
Michael, Gabriel, and Raphael: genderless archanges, they/he/she pronouns.

Ba’al and other monsters: Many of the monster from The Last Halloween do not appear to have gender, and Ba’al used they pronouns
The Bandits from Rock and Riot. A gang of non-binary kids in the 1950s.
BLL: witch with anthropomorphic traits from some short comics by @solomonfletcher
Cayli: agender friend of one of the main characters of Always Human (@alwayshumancomic)
Dad: father of the protagonist of The Last Halloween. I literally can’t remember if we’ve learned their name. They are gender non-conforming and use they pronouns.
Dee & Dum from Alice and the Nightmare are non-binary
Ember: a non-binary human magic user (Orphic) from @blindsprings. Also check out the non-binary (genderless?) spirits.
Eth: a non-binary human in a fantastic version of British Columbia from @eths-skin (pictured above with a squirmy seal)
The Fairy Warriors: A group of fairies and a witch, all of whom are non-binary!
Fenix-from Pschonaut. An asexual, genderqueer, alien kid
Goodbye to Halos is a webcomic with several trans characters and plans to introduce more. Two non-binary characters introduced so far are:

Jessica the Bear Bandit is non-binary.
Leo the Lion is genderqueer and still exploring his gender.

Helly from White Noise, a webcomic/book (there is a written version of each page) about war and prejudice against magical species. Helly’s species doesn’t have gender.
Irma/Irving/Ir* from The Princess, is also gender-fluid, we think. We’re not entirely sure because Ir* is still kinda developing their identity, being a young kid and all. But the comic’s main character, Sarah, knows very well that she is a girl and will not accept anyone telling her that she can’t know that just because she’s a child. Anyway lovely trans kids all around, it’s beautiful. Written by a trans woman who wished she had a comic like that when she was Sarah’s age.
JD: friend of the main character of Shades of A: a fat, kinky, exuberant enby.
@jobsatisfactioncomic is a comic about a professional summoner, their assistant, and some demons. The genders of the demons are all listed as ???. Also:

Gaspar Villalba de Gallo is a genderfluid summoner.
Lemme Laviolett is a non-binary assistant to the summoner.
Sinh Thuy is a non-binary summoner.

Jordan Davignon : from Jenny Haniver, a trans feminine band member who uses fey and jer pronouns.
Khut: one of the main characters of Star Trip, a shapeshifting alien who uses they pronouns. “They” is the default pronoun used in the intergalactic community. Another other apparently non-binary character is, OdoZun: two alien creatures who are bound to each other.
Kylie: from Rain (a slice of life comic about a trans woman), a genderfluid friend of the main character
Lavender: a fairy from Peritale, who uses they/them pronouns.
Louise: A human from Monsterkind that goes by they pronouns.
Lucy: a trans feminine wizard from Never Satisfied. (also look for Tetsu, who uses they pronouns)
Magpie: aka Maggie, a shape-changing bird person from Sister Claire (pictured below). The comic also includes:

Grimm: A strange cat (or is it?).

Marco from Kimchi Cuddles, an educational slice-of-life comedy about polyamoury, is gender-fluid.
Mason is a nonbinary person in Miriam Beach.
Mickey is a weredog that uses they/them pronouns in The Last Halloween.
MinorityMonster: includes several non-binary monster characters in one-shot comics
Nails and Teeth: I’m not familiar with the comic, but there are several non-binary, monstrous characters.
Neokosmos: According to fans, no one in this comic is cis. There are several characters that we have more details about:

Tye and Z: Human childred-raised genderless -they pronouns
Iris: Human child-raised genderless but transitioned to female (not non-binary)
Xaveria and Zehra: Aliens (Compies) that are monogender and use they pronouns most of the time, but use she in intimate contexts.

Neve from Ignition Zero is gender-fluid, but they also are a drag queen! And they get along really well with fairies and spirits, even the dangerous ones, which is kind of the main theme of this comic. Also the comic’s main character is a lovely gay ace boy and the author is actually genderfluid.
Nokia: main character from Immortal Nerd, a comedy/SF webtoon about a brightly-colored future where gender diversity is respected.
Norn: a human with monstrous qualities from Demon Street (@demonstreet). Uses they pronouns.
Pat: a young alien golem from Mashup
River: a cutie genderfluid dog person from 13 Fireflies
RJ: From the webcomic Paranatural, a human who “Eschews school rules, public speaking, the gender binary.”
Roji is the a cat that goes by they/them.
San: the cyber spirit from @dragonhusbandscomic does not have gender and uses it pronouns.
Shannon ‘Shaz’ Arcuni- A ghoul from Bloody Urban. I don’t know much about them!
Taisce Brennan is a selkie who uses they/them pronouns in Skyvein.
Tanin: from @daimonikoscomic is a non-binary demon.
Tedd: from El Goonish Shive, a genderfluid teen dealing with strange and supernatural situations.
Valley of the Silk Sky: (@valleyofthesilksky) Filled with non-binary aliens and at least one non-binary human, including:

Halvanylila: alien, not a great hunter
Razi: human, uses ze/zer pronouns

Untamo: from Prague Race, a humanoid monster who canonically uses they/them pronouns.
Zephyr is a nonbinary alien from Log Date.

Print Comics

Crona: from Soul Eater. In the original Japanese, their gender is never specified, and some fans consider them non-binary.
Desire: from the Sandman series, a personification of desire who is referred to as a he-she and sister-brother.
Hanji Zoe from Attack on Titan (an anime and manga) has no confirmed canon gender and is thought to be non-binary.
Loki: shapeshifter and confirmed genderqueer god of stories (or tricks, or lies, depending on the story line) in the Marvel universe (we can probably assume movie-Loki is genderqueer too. At least I hope so).
Petalwing: A fairy-like creature from ElfQuest who goes by they or it pronouns. Their whole species (Preservers) are sexless and genderless. Other Perservers are Berrybuzz, Tickletoe, and Willowsnap.
Porcelain: from DC’s Secret Six is confirmed genderfluid. The character has said, “some days I feel like a girl, and other days not-so-much.”
Xavin: A Skrull from Marvel comic, Runaways. Skrulls are shapeshifters, and Xavin comfortably changes sex/gender (and is in a lesbian relationship with another one of the Runaways).
Yasel: non-binary sibling of one of the main characters of Always Human (@alwayshumancomic)

Games

Aetherborn from Magic the Gathering are non-binary and use they pronouns. Aetherborn are creatures that are created from Aether.
Amaterasu: a Japanese goddess in the form of a white wolf, from the game Okami. In some versions is genderless and is referred to by it, she, and he pronouns.(below)
Asari: ‘a mono-gendered species with no concept of gender differences. According to Liara, "male and female have no real meaning for us,” and, if asked, says that she is “not precisely a woman.”’
Ashiok: From Magic the Gathering, a nongendered Planeswalker.
Bugs n Kisses: the protagonist can use they/them pronouns
Cloud of Darkness from Final Fantasy III is a genderless being that often takes a feminine form and uses we pronouns.
Eldrazi: a race from Magic the Gathering. They travel between planes devouring their life energies. They have been assigned genders by others, but may not have gender of their own (according to head designer Mark Rosewater).
Hallar is a Llanowar elf from Magic the Gathering, who uses genderless pronouns in their language.
Kindred: From League of Legends, the Kindred Champion is an archer refereed to with they pronouns, usually. They have two parts: the wolf which is sometimes referred to with he pronouns, and the lamb referred to with she pronouns. They are identified as bigender by fans.
Leo Kliesen from Tekken has an ambiguous gender.
Luna from the tabletop RPG Exalted. They are a trickster god who appears equally male and female. She is also the mother of the Lunar Exalted.
Monster Mountain is an app that has several non-binary monsters. screen shot of the ones available at this time:

Pokemon: Several pokemon are genderless, particularly legendaries and pokemon that are similar to machines and other non-living objects (above are Mewtwo and Mew, both genderless).
The Pyro: from Team Fortress 2 has no stated gender
Scribbles: a Fae dragon from Flight Rising. They have been described as transcending gender, and use they/them pronouns.
Subaru Kojou: from Sakura Wars: So Long My Love.
Turing: from Read Only Memories, is a gender-questioning robot who prefers they/them pronouns. The game also includes:

TOMCAT: An androgynous hacker who uses they pronouns.
Sky: A minor character that uses they pronouns.

Undertale: potentially has several non-binary characters witch are referred to with they pronouns (the links that follow contain spoilers):

The Protagonist: a human child
Monster Kid: a monster that follows the protagonist at one point.
Napstablook: a sad ghost.

Zer0: from Borderlands, an assassin with mysterious origins and ambiguous gender.

Books

Alex: a genderfluid shifter from Static. Uses he/she pronouns depending on presentation, though their gender presentation doesn’t always match up with their gender identity. The book also includes other shapeshifting non-binary characters.
Bel Thorne: A mercenary officer in some of Lois McMaster Bujold’s Vorkosigan Saga. Uses it pronouns, and its presentation shifts between masculine and feminine depending on who it’s attracted to. The author uses the h-slur to describe it.
Shadow: a teenage half-demon, trans feminine, agender, superhero from the ebook To Stand in the Light.
Person of Indeterminate Gender: A henchperson from A Series of Unfortunate Events (probably not a positive character).

Podasts

The Adventure Zone is a family playing Dungeons and Dragons and frequently uses the singular they for characters.

Roswell is a red clay construct/earth elemental and also a vermilion flycatcher that seems to be genderless.
Taako is an elf and one of the player characters. Confirmed queer, but not confirmed non-binary, though the character seems to hint at that (uses he pronouns).
The Void Fish is a giant galaxy jellyfish that doesn’t appear to have a gender.

Welcome to Night Vale is radio show style podcast

Angels all named Erika seem to be genderless with fluid gender presentation. “Angels do not hear gender,” -The Debate
Sheriff Sam is presumably human and sheriff of the secret police. They use singular they pronouns.

Movies

Biaggio: from the movie The Kings of Summer says he doesn’t see himself as having a gender (he does not appear to be a positive character).
Jeriba “Jerry” Shigan: from the movie Enemy Mine. Shiban is a Drac, an alien race that reproduce asexually and apparently have no gender identity (content warning for h-slur).

Other lists of non-binary characters in fiction:

Fictional Depictions of Nonbinary Gender from nonbinary.org

Genderless worlds in scifi on wikipedia (cw for potentially cissexist language and use of the h-slur)

Please feel free to send asks or submissions if you have anything to add!